Rapid molecular detection of Senecavirus A based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and CRISPR/Cas12a
In summary, the RT-LAMP-Cas12a-FQ and RT-LAMP-Cas12a-FB methods developed are promising for early detection and routine surveillance of porcine SVA in resource-poor areas.
Authors
- Rongxia Guo
- Jincai Yang (ORCID: https://orcid.org/0000-0002-4446-5809)
- Huibao Wang
- Ping Liu (ORCID: https://orcid.org/0000-0002-0800-5303)
- Canhua Jiang (ORCID: https://orcid.org/0000-0002-8133-1981)
- Xiaoming Li (ORCID: https://orcid.org/0000-0002-5743-1579)
- Jing Wang (ORCID: https://orcid.org/0000-0001-9761-9915)
- Ruifu Yang (ORCID: https://orcid.org/0000-0003-3219-7269)
- Yanyan Chang (ORCID: https://orcid.org/0000-0002-2271-4456)
- Qiaoying Zeng (ORCID: https://orcid.org/0000-0001-9416-2075)
Institutions
- Gansu Agricultural University (CN)
- Lanzhou University of Technology (CN)
- Gansu Great Wall Electrical and Electronics Engineering Research Institute (CN)
- Gansu Institute of Mechanical and Electrical Technology (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-04-04
- DOI
- https://doi.org/10.3389/fbioe.2025.1451125
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81