Scale-up of spirulina protein extraction process for pilot reactor design

This investigation focuses on designing a 400 L pilot reactor for extracting proteins from Spirulina using the method of scale-up dimensionless numbers. The first step was to determine the factors to study the extraction at 0.5 L, such as temperature, time, and agitation. Then, by using statistical methods, we identified the optimal extraction yield point. Subsequently, at the 4 L scale, we tested different impellers to find specific dimensionless number ratios, which were useful in designing the 400 L reactor, in addition to estimating stirring speeds for maximum protein extraction. The next step in this process is the isolation of protein.