Production of fusel alcohols and fusel acetates by pathogenic fungi in the Ceratocystidaceae

The family Ceratocystidaceae includes economically important plant pathogens that vary in host preference and lifestyle. These fungi are believed to attract insect vectors, for their dispersal through their floral and fruity scents. This study aimed to identify the volatiles produced by a subset of fungi within the Ceratocystidaceae using gas chromatography coupled with mass spectrometry. The primary volatiles produced by most genera in the family were fusel alcohols and fusel acetates, but their emission rates differed significantly between genera and isolates from a single species. Ceratocystis albifundus collected from Protea cynaroides produced higher levels of fusel acetates compared to isolates from Terminalia sericea . In addition, significant differences in volatile biosynthesis were observed between isolates grown under different temperatures. Results of this study demonstrate that Ceratocystidaceae exhibit varied volatile profiles, but further research is needed to understand the ecological and physiological mechanisms underlying this plasticity. • Fungi in the Ceratocystidaceae emit fusel alcohols and acetates to attract insect vectors. • Different genera of the Ceratocystidaceae vary in their production of fusel alcohols and fusel acetates. • Isolates of C. albifundus from two different plant hosts produced different quantities of volatiles. • Isolates of C. albifundus produced different volatile profiles when incubated at different temperatures.