Impact of heterologous expression of Cannabis sativa tetraketide synthase on Phaeodactylum tricornutum metabolic profile
Our study demonstrates the successful expression of cannabinoid biosynthetic genes in P. tricornutum but highlights challenges in OA accumulation, likely due to endogenous metabolic interactions. These findings underscore the complexity of metabolic engineering in diatoms and suggest the need for further pathway optimization and metabolic flux analysis to achieve efficient cannabinoid biosynthesis. This research contributes to advancing sustainable biotechnological approaches for cannabinoid production.
Authors
- Isabel Desgagné‐Penix (ORCID: https://orcid.org/0000-0002-4355-5503)
- Karen Cristine Gonçalves dos Santos (ORCID: https://orcid.org/0000-0003-3270-3133)
- Fatma Meddeb‐Mouelhi (ORCID: https://orcid.org/0000-0002-2071-0486)
- Hugo Germain (ORCID: https://orcid.org/0000-0002-7046-6194)
- Elisa Fantino (ORCID: https://orcid.org/0000-0001-8393-3241)
- Sarah‐Eve Gélinas (ORCID: https://orcid.org/0009-0009-3030-835X)
- Fatima Awwad (ORCID: https://orcid.org/0000-0003-0428-5552)
- Natacha Mérindol (ORCID: https://orcid.org/0000-0001-6235-6465)
- Alexandre Custeau
- Nicolas Sene
Institutions
- Université du Québec à Trois-Rivières (CA)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2025-04-04
- DOI
- https://doi.org/10.1186/s13068-025-02638-1
- Citations
- 2
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Canada Research Chairs
- Mitacs