In vitro and in vivo evaluation of anti-HER2 antibody conjugates labelled with 225Ac
During this study, the optimization of preparation and radiolabelling of HER2 targeting antibodies with <sup>225</sup>Ac was accomplished. Furthermore, the radioconjugate <sup>225</sup>Ac-DOTA-pertuzumab was prepared and evaluated for the first time. The radioconjugates of both tested antibodies demonstrated excellent qualities in terms of stability and HER2 receptor affinity. Initial ex vivo studies indicated that especially the radioconjugate <sup>225</sup>Ac-DOTA-pertuzumab is a very promising candidate for further more detailed in vivo studies.
Authors
- Marián Hajdúch (ORCID: https://orcid.org/0000-0002-4834-908X)
- Martin Vlk (ORCID: https://orcid.org/0000-0003-1044-1910)
- Kateřina Ondrák Fialová (ORCID: https://orcid.org/0000-0001-7317-1503)
- Ján Kozempel (ORCID: https://orcid.org/0000-0002-6315-9225)
- Alfred Morgenstern (ORCID: https://orcid.org/0000-0002-7208-3680)
- Frank Bruchertseifer (ORCID: https://orcid.org/0000-0003-2125-4483)
- Marek Pruszyński (ORCID: https://orcid.org/0000-0001-8835-2462)
- Lukáš Ondrák (ORCID: https://orcid.org/0000-0001-8070-8081)
- Zbyněk Nový (ORCID: https://orcid.org/0000-0002-7716-2142)
- Miloš Petřík (ORCID: https://orcid.org/0000-0003-1334-5916)
- Kateřina Nováková (ORCID: https://orcid.org/0000-0002-5597-5361)
- Katarína Hajduová (ORCID: https://orcid.org/0009-0000-3078-0907)
Institutions
- National Centre for Nuclear Research (PL)
- Instytut Chemii i Techniki Jądrowej (PL)
- Joint Research Centre (DE)
- Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry (CZ)
- Institute of Molecular and Translational Medicine (CZ)
- Czech Technical University in Prague (CZ)
- Palacký University Olomouc (CZ)
Publication Details
- Journal
- EJNMMI Radiopharmacy and Chemistry
- Published
- 2025-04-04
- DOI
- https://doi.org/10.1186/s41181-025-00337-8
- Citations
- 2
- Primary Topic
- Radiopharmaceutical Chemistry and Applications
- Type
- article
- Field-Weighted Citation Impact
- 2.72
Funders
- České Vysoké Učení Technické v Praze
- European Commission
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Technologická Agentura České Republiky
- Agentura Pro Zdravotnický Výzkum České Republiky
- Nuclear Physics