Estimating malaria antigen dynamics and the time to negativity of next-generation malaria rapid diagnostic tests
In the detection of P. falciparum malaria, LDH versus HRP2-based RDTs had a faster TTN due to a combination of lower accumulated antigen concentrations and faster decay rates, even for more sensitive LDH-based RDTs. Detection of LDH versus HRP2 by RDT is more likely to reflect a new or very recent infection. For NG-RDTs that target both antigens, HRP2 is likely to contribute more to the test signal than LDH in recently treated infections unless the infection has hrp2/hrp3 gene deletions. Antigen decay data combined with analytical sensitivity contributes to understanding RDT performance and interpretation.
Authors
- Rebecca Barney (ORCID: https://orcid.org/0000-0002-9851-6659)
- Davis Ropafadzo Mumbengegwi (ORCID: https://orcid.org/0000-0001-9176-904X)
- Gonzalo J. Domingo (ORCID: https://orcid.org/0000-0002-1004-4395)
- Allison Golden (ORCID: https://orcid.org/0000-0001-6891-5176)
- William Sheahan (ORCID: https://orcid.org/0000-0002-4192-4491)
- Ihn Kyung Jang (ORCID: https://orcid.org/0000-0001-8899-7930)
- Xue Wu (ORCID: https://orcid.org/0009-0005-0730-0406)
- Henry Ntuku (ORCID: https://orcid.org/0000-0001-7218-2710)
- Brooke Whittemore (ORCID: https://orcid.org/0000-0003-3871-6111)
- Hannah Slater (ORCID: https://orcid.org/0000-0003-4291-7899)
- Lucille Dausab
- Michelle S. Hsiang (ORCID: https://orcid.org/0000-0003-1217-2742)
- Smita Das (ORCID: https://orcid.org/0000-0002-4834-6436)
Institutions
- Program for Appropriate Technology in Health (US)
- University of California, San Francisco (US)
- University of Namibia (NA)
- Seattle University (US)
- The University of Texas Southwestern Medical Center (US)
Publication Details
- Journal
- Malaria Journal
- Published
- 2025-04-04
- DOI
- https://doi.org/10.1186/s12936-025-05350-5
- Citations
- 3
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 5.64
Funders
- Bill and Melinda Gates Foundation