Exploration of alternative microfiltration modalities for the harvest and clarification of diverse recombinant proteins from high-density <i>E. coli</i> culture and lysate using hollow fibre, flat sheet cassette, and vibro membrane filtration technol
High-density E. coli microfiltration and lysate clarification were tested for three diverse recombinant proteins, where hollow fibre and vibro membrane filtration outperformed flat sheet cassette in terms of process time, suspended solid loading, and target protein recovery.
Authors
- Calvin Adams
- Cédric Charretier
- Analyn Yu
- A. Luo (ORCID: https://orcid.org/0009-0006-3051-2236)
- Tao Yuan (ORCID: https://orcid.org/0009-0006-9530-207X)
- Joyce Ni
- Jennifer Reid (ORCID: https://orcid.org/0000-0002-7046-5271)
- Patrícia Gomes‐Alves (ORCID: https://orcid.org/0000-0001-7245-6785)
- Boon Hong Kong (ORCID: https://orcid.org/0000-0003-3255-9960)
- A. Hesti Wulandasari Amir
- A. CHEN
- Neveathan Jeyachandran
- Xavier Teixeira
- Andrew Szto
Institutions
- Center for Global Development (US)
- Sanofi (France) (FR)
Publication Details
- Journal
- Journal of Industrial Microbiology & Biotechnology
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1093/jimb/kuaf008
- Citations
- 1
- Primary Topic
- Membrane Separation Technologies
- Type
- article
- Field-Weighted Citation Impact
- 0.20
Funders
- Sanofi