Development and validation of optimized lentivirus-like particles for gene editing tool delivery with Gag-Only strategy
In conclusion, this optimized platform effectively encapsulates CE-8e-SpRY mRNA and sgRNA, achieving high editing efficiencies across multiple genes and cell types. We introduce a safer and more efficient gene editing tool delivery system by constructing LVLPs based on the Gag-Only strategy. Our study presents a promising implication for cancer immunotherapy.
Authors
- Xiaohua Jin (ORCID: https://orcid.org/0000-0001-9249-7496)
- Xu Ma (ORCID: https://orcid.org/0000-0001-7674-3589)
- Yanzhe Hao (ORCID: https://orcid.org/0000-0002-0111-9826)
- Jinlin Jia
- Pengfei Zhang (ORCID: https://orcid.org/0000-0003-4437-8112)
- Hu Wang
- Lisha An
- Xiaofang Cao
- Qi Ma
Institutions
- Chinese Center For Disease Control and Prevention (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
- Genetic Resources Center (JP)
- National Institute for Viral Disease Control and Prevention (CN)
- National Institute for Communicable Disease Control and Prevention (CN)
- Tianjin Municipal Research Institute for Family Planning (CN)
Publication Details
- Journal
- European journal of medical research
- Published
- 2025-04-04
- DOI
- https://doi.org/10.1186/s40001-025-02499-2
- Citations
- 8
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 14.54
Funders
- National Key Research and Development Program of China