Chromosome-level genome assembly of the crofton weed (Ageratina adenophora)
Crofton weed (Ageratina adenophora), a significant invasive species, extensively disrupts ecosystem stability, leading to considerable economic losses. However, genetic insights into its invasive mechanisms have been limited by a lack of genomic data. In this study, we present the successful de novo assembly of the triploid genome of A. adenophora, leveraging long-read PacBio Sequel, optical mapping, and Hi-C sequencing. Our assembly resolved into a haplotype-resolved genome comprising 51 chromosomes, with a total size of ~3.82 Gb and a scaffold N50 of 70.8 Mb. BUSCO analysis confirmed the completeness of 97.71% of genes. Genome annotation revealed 3.16 Gb (76.44%) of repetitive sequences and predicted 123,134 protein-coding genes, with 99.03% functionally annotated. The high-quality reference genome will provide valuable genomic resources for future studies on the evolutionary dynamics and invasive adaptations of A. adenophora.
Authors
- Guifen Zhang
- Fuyan Liu (ORCID: https://orcid.org/0000-0001-8693-7926)
- Tingting Li (ORCID: https://orcid.org/0000-0001-5727-2179)
- Yibo Zhang
- Wanxue Liu (ORCID: https://orcid.org/0000-0002-6069-525X)
- Yan Pan (ORCID: https://orcid.org/0000-0002-6870-3015)
- Lin Du (ORCID: https://orcid.org/0000-0002-2070-2021)
- Weining Song (ORCID: https://orcid.org/0000-0003-2052-3880)
- Ying Zhang (ORCID: https://orcid.org/0000-0001-8988-1649)
- Bo Liu (ORCID: https://orcid.org/0000-0002-7840-9450)
- Hailing Wang (ORCID: https://orcid.org/0000-0002-6976-8806)
- Ruiying Li (ORCID: https://orcid.org/0009-0008-4179-8246)
- Jianyang Guo
- Fanghao Wan (ORCID: https://orcid.org/0009-0004-4877-8345)
Institutions
- Beijing VDJBio (China) (CN)
- North West Agriculture and Forestry University (CN)
- Agricultural Genomics Institute at Shenzhen (CN)
- Institute of Plant Protection (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- University of Chinese Academy of Sciences (CN)
- Jiangxi Agricultural University (CN)
- Northwest A&F University (CN)
Publication Details
- Journal
- Scientific Data
- Published
- 2025-04-02
- DOI
- https://doi.org/10.1038/s41597-025-04637-1
- Citations
- 2
- Primary Topic
- Genetic diversity and population structure
- Type
- article
- Field-Weighted Citation Impact
- 3.63
Funders
- State Administration of Foreign Experts Affairs
- National Key Research and Development Program of China