Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors

Our findings demonstrate that the T6SS can be engineered to deliver both canonical and noncanonical effectors, providing a robust platform for targeted antibacterial and antifungal applications. The modularity of the system enables precise pairing of effectors with structural components such as VgrG and PAAR proteins, optimizing delivery efficiency. These engineered systems provide new opportunities for the development of biocontrol strategies in agriculture, microbiome modulation, and potential therapeutic applications. Future advancements in bioinformatics and protein engineering will further increase the specificity and functionality of T6SS-based delivery systems, offering innovative tools for managing microbial ecosystems and addressing global challenges in health and agriculture.