Determination of the Metabolic Mechanism of the Principal Components From <scp><i>Moringa oleifera</i></scp> (Lam) Seed by MALDI‐IMS

In this study, the components of Moringa oleifera (Lam) seed were extracted using an ultrasonic-assisted extraction technique with 70% methanol solution as the solvent. The antioxidant properties of the extract were evaluated through its scavenging abilities against DPPH^. and ABTS. The antiproliferative effects of this extract on breast cancer MDA-MB-231, colon cancer SW480, leukemia HL-60, liver cancer SMMC-7721, and lung cancer A549 were assessed using the MTS assay. The distribution of MA, D-trehalose, and rbGlu in the heart, kidney, liver, and spleen of mice in various intervals was visually investigated using MALDI-IMS. In particular, the metabolic pathways of rbGlu were further elucidated. The result shows that rbGlu is metabolized to rbot-Glu in mice within 1 h. This approach further substantiates the use of MALDI-MSI technique in situ for studying the pharmacological mechanisms of bioactive component in natural products.