Immobilization of alginate C-5 epimerases using <i>Bacillus subtilis</i> spore display

Seaweed is a scalable resource that requires no fresh water, fertilizer, or arable land, making it an important biomass for bioeconomies. Alginates are a major component of brown seaweed and are widely used in food, feed, technical, and pharmacological industries. To tailor the functional properties of alginates, alginate epimerases have shown to be promising for postharvest valorization of alginate. This study investigates an efficient and easy method to produce immobilized alginate epimerases, thus opening new industrial use cases. In this study, the alginate epimerases are immobilized on the surface of <i>Bacillus subtilis</i> spores. The bacterium forms spores in reaction to nutrient starvation, which are highly resistant to external influences and can be repurposed as a stable protein display platform for numerous applications due to its ease of genomic manipulation and cultivation.