EXPLORING THE POTENTIAL OF SHOUCHELLA OSHIMENSIS P-106 FOR CYCLODEXTRIN GLUCANOTRANSFERASE PRODUCTION

Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an enzyme that utilizes starch by promoting an intramolecular transglycosylation reaction to produce cyclodextrins. An alkaliphilic, spore-forming, Gram-positive, potential CGTase producing bacteria was isolated from maize field and was identified as Shouchella oshimensis P-106 by 16S rDNA sequencing. The isolate P-106 efficiently produced 1.37 U/ml of β-CGTase as a major product and showed little existence of α- and γ-CGTase production. Optimization by one-factor-at-a-time (OFAT) approach significantly enhanced the β-CGTase production to 2.31 U/ml showed 1.68-fold increase.