Immobilization of Lactase Enzyme on Aleppo Bentonite

This research was conducted at Idlib University, located in northwestern Syria, during the years 2023 and 2024. Lactase was immobilized on Aleppo Bentoniteby adsorption. A surfactant (SDS) was added, and the immobilization yield, immobilization efficiency, enzyme recovery rate, temperature, and optimum pH were studied. The results demonstrated the success of the lactase enzyme immobilization process on Aleppo Bentonite. They also demonstrated that adding a surfactant (SDS) increased the efficiency of the enzyme immobilization process. The Aleppo Bentonite treated with 0.5 N hydrochloric acid using a magnetic stirrer was the best, with an immobilization yield of 90%. The optimum pH for enzyme immobilization was 6.6, and the immobilized enzyme demonstrated greater resistance to pH drops in acidic media. The optimum temperature for enzyme immobilization was 70°C. The results also showed that the immobilized enzyme demonstrated greater stability at high temperatures and was capable of hydrolyzing 80% of lactose after three hours. Furthermore, the immobilized enzyme retained 60% of its original activity after six consecutive cycles. The study concluded that activated Aleppo Bentoniteis a good support for β-galactoside enzyme immobilization.