Decomposition of Overlapping and Touchingm-Fish Chromosomes Using Image Process Techniques

Automatically segmenting partially obscured and touching objects is an extremely challenging task. Chromosome imaging may be impacted by partial blockage and chromosomal contact. This is one of the primary obstacles to automating the analysis. Many segmentation (decomposition) approaches have been developed for typical banded chromosomal images. With differing degrees of success, some of these strategies only address touching situations, while others address both. Most techniques employ simply the skeleton, convex hulls, and curvature of chromosomal clusters as geometry information. The geometry-based methods only look at the form of the boundary of a chromosomal cluster. Even while the border shape provides a wealth of information on the cluster formation, it is often insufficient in certain cases, such as when two chromosomes touch by their short or long sides to form a long or thick chromosome. These touching cases are easily recognised when the pixelmemberships are displayed by two distinct hues, like in M-FISH.