De Novo transcriptome assembly and annotation of the wild service tree Sorbus torminalis (L.) Crantz

An RNA-seq library was generated from pooled RNA extracted from four different tissues (young leaves, mature leaves, stems and roots) of a single S. torminalis sapling and sequenced on the Illumina HiSeq 2500 platform. This dataset was used to produce and annotate the first transcriptome assembly for S. torminalis, paving the way for future genomic research and applications in this species. For example, this study will facilitate the development of molecular markers such as SSRs and SNPs, useful for genetic studies, breeding programs, and conservation efforts and will serve as a reference for future differential expression studies, providing a deeper understanding of the species' biology.

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Publication Details

Journal
BMC Research Notes
Published
2025-04-02
DOI
https://doi.org/10.1186/s13104-025-07163-w
Citations
1
Primary Topic
Botanical Studies and Applications
Type
article
Field-Weighted Citation Impact
1.75

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De Novo transcriptome assembly and annotation of the wild service tree Sorbus torminalis (L.) Crantz

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BMC Research Notes
Botanical Studies and Applications
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article

De Novo transcriptome assembly and annotation of the wild service tree Sorbus torminalis (L.) Crantz

Ilaria Spanu, Francesca Bagnoli, Andrea Piotti, Giovanni G. Vendramin, Camilla Avanzi, Sara Pinosio
article en
1 citations

Abstract

An RNA-seq library was generated from pooled RNA extracted from four different tissues (young leaves, mature leaves, stems and roots) of a single S. torminalis sapling and sequenced on the Illumina HiSeq 2500 platform. This dataset was used to produce and annotate the first transcriptome assembly for S. torminalis, paving the way for future genomic research and applications in this species. For example, this study will facilitate the development of molecular markers such as SSRs and SNPs, useful for genetic studies, breeding programs, and conservation efforts and will serve as a reference for future differential expression studies, providing a deeper understanding of the species' biology.

BMC Research NotesVol. 18(1)
Institute of Biosciences and Bioresources (IT)
H2020 Societal Challenges
Zero hunger
Openalex Percentile: Top 16%
Botanical Studies and Applications
1.75
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