Three-dimensional imaging and computational quantitation as a novel approach to assess nerve fibers, enteric glial cells, mast cells, and the proximity of mast cells to the nerve fibers in human sigmoid mucosal biopsies from healthy subjects
The novel approach enables faster tissue clearing/immunolabeling, high-quality 3D imaging and precise computational quantitation of NFs, cells and proximity of MCs to NFs in human sigmoid biopsies which may allow new insight to detect alterations in colonic-related diseases.
Authors
- Yvette Taché (ORCID: https://orcid.org/0000-0002-1044-5279)
- Jessica Sohn
- Pu‐Qing Yuan (ORCID: https://orcid.org/0000-0002-0067-510X)
- Swapna Mahurkar‐Joshi (ORCID: https://orcid.org/0000-0001-9591-4590)
- Tao Li (ORCID: https://orcid.org/0000-0002-9174-1271)
- Lin Chang
Institutions
- Center for Digestive and Liver Diseases (US)
- VA Greater Los Angeles Healthcare System (US)
Publication Details
- Journal
- Journal of Neuroscience Methods
- Published
- 2025-04-03
- DOI
- https://doi.org/10.1016/j.jneumeth.2025.110436
- Citations
- 2
- Primary Topic
- Gastrointestinal motility and disorders
- Type
- article
- Field-Weighted Citation Impact
- 3.62
Funders
- National Institutes of Health