Effect of pyriproxyfen on biological parameters and morphometry of Aedes aegypti Linnaeus, 1762 (Diptera: Culicidae) in the city of Manaus, Amazonas

In Brazil, Aedes aegypti is the primary vector of arboviruses, and its control relies mainly on insecticide use. However, excessive application on these products has led to the selection of resistant populations. To address this challenge, products like Pyriproxyfen (PPF), an insect growth regulator, have been used as a viable alternative. In this context, the present study evaluated the susceptibility, resistance ratio, and biological changes of A. aegypti exposed to PPF. Samples were collected in eight neighborhoods using oviposition traps, and the Rockefeller strain was used as a susceptibility standard. Fecundity tests recorded a hatching rate of 72.4 % in the PPF groups, compared to 89.2 % in the control (p < 0.05), and fertility of 72.4 % for PPF compared to 89.2 % in the control (t = 204.5, df = 4, p < 0.05). Longevity was assessed in males, females, and couples (χ² = 20.35, df = 5, p > 0.05). Wing morphometric analyses were reinforced by Mahalanobis distance (1.7127; p < 0.001), Procrustes analysis (0.0064; p = 0.5027), and canonical variable analyses. The width of the cephalic capsules was greater in mosquitoes exposed to PPF (Mann-Whitney U = 369; p < 0.0099). The emergence inhibition rate ranged from 65.33 ± 4 to 100 ± 0 for the Rockefeller strain and from 59.33 ± 4 to 88.66 ± 2 for the field population, with a resistance ratio of 0.68. The study concludes that the A. aegypti population in Manaus, remains susceptible to PPF, and the observed alterations were not significant enough to compromise the vector's biology.