Enhanced Natamycin production in Streptomyces gilvosporeus through phosphate tolerance screening and transcriptome-based analysis of high-yielding mechanisms
Our findings suggest that the high production performance of GR-2 is primarily due to the upregulation of the natamycin biosynthetic gene cluster and genes related to precursor supply. Increasing the intracellular supply of valine and glutamate significantly enhanced natamycin production. Additionally, the natamycin biosynthetic gene cluster is likely positively regulated by PhoP and PhoR. Our work presents a novel strategy for strain screening and evolution to improve natamycin production and identifies novel molecular targets for metabolic engineering.
Authors
- Xusheng Chen (ORCID: https://orcid.org/0000-0002-6157-8565)
- Ting Qiu (ORCID: https://orcid.org/0009-0007-8209-4424)
- Wen Xiao (ORCID: https://orcid.org/0000-0002-4951-7022)
- Liang Wang (ORCID: https://orcid.org/0000-0001-5339-7484)
- Jianhua Zhang (ORCID: https://orcid.org/0000-0002-3819-2437)
- Hongjian Zhang (ORCID: https://orcid.org/0000-0002-1937-2766)
Institutions
- Jiangnan University (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-04-02
- DOI
- https://doi.org/10.1186/s12934-025-02696-y
- Citations
- 4
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 5.58
Funders
- Jiangsu Agricultural Science and Technology Innovation Fund
- Government of Jiangsu Province
- Key Laboratory of Industrial Biotechnology
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities