Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease (CMD)
Cassava is an important staple crop in tropical and subtropical regions. Cassava mosaic disease (CMD) is one of the most dangerous diseases affecting cassava production in Africa. Since the first reported in Southeast Asia in 2015, the CMD prevalence has become a concern in Southeast Asia. To combat it, CMD resistance has been introduced from African cassava into Asian elite cultivars. However, efficient DNA markers for the selection of CMD resistance are not available. The <i>CMD2</i> locus confers resistance to African cassava mosaic virus via non-synonymous substitutions in the DNA polymerase δ subunit 1 gene (<i>MePOLD1</i>). Here, we developed DNA markers to identify the mutations providing the resistance. We examined the association between the resistance score in CMD-infected fields and the genotypes of hybrids of CMD-resistant and ‑susceptible Asian lines. Our study provides powerful tools to the global cassava breeding community for selecting CMD resistant cassava.
Authors
- Nguyễn Thị Huyền Trang (ORCID: https://orcid.org/0000-0002-9471-2660)
- Hiroki Tokunaga (ORCID: https://orcid.org/0000-0001-7316-0791)
- Nguyen Hai Anh
- Nguyen Ba Tung (ORCID: https://orcid.org/0000-0002-5888-4641)
- Cu Thi Le Thuy
- Xiaofei Zhang (ORCID: https://orcid.org/0000-0003-4516-9179)
- Phạm Thị Thanh Nhàn (ORCID: https://orcid.org/0000-0003-1819-2840)
- Motoaki Seki (ORCID: https://orcid.org/0000-0001-8288-0467)
- Lê Huy Hàm
- Pham Thi Huong (ORCID: https://orcid.org/0000-0001-7264-1200)
- Lê Thi Huong (ORCID: https://orcid.org/0000-0003-1123-2037)
- Truong Minh Hoa
Institutions
- Kihara Institute for Biological Research (JP)
- Pioneer (United States) (US)
- Japan International Research Center for Agricultural Sciences (JP)
- RIKEN Center for Sustainable Resource Science (JP)
- International Center for Tropical Agriculture (KE)
- Plant (United States) (US)
- Agricultural Genetics Institute (VN)
- Agricultural Research Center (EG)
- University of California, Davis (US)
- Yokohama City University (JP)
Publication Details
- Journal
- Breeding Science
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1270/jsbbs.24046
- Primary Topic
- Cassava research and cyanide
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan International Cooperation Agency
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- Science and Technology Research Partnership for Sustainable Development