Genome-wide identification and functional analysis of the longan CONSTANS (CO) family

Longans are among the most economically important subtropical fruits. Its flowering is sensitive to the photoperiod, and flowering time has a significant influence on yield and quality. CONSTANS-like (COL) gene plays a key role in regulating induced flowering in longans. However, the specific role of the COL gene family in the regulation of flowering remains unknown. In this study, 10 DlCOL genes were identified in longans using comprehensive bioinformatics analysis and named based on their physical chromosomal locations. Phylogenetic tree analysis showed that DlCOL genes were divided into three subfamilies, each with a conserved domain. When combined with collinearity analysis, we found DlCOL genes were more closely related to COL genes of dicotyledons. DlCOL family genes are differentially expressed in various longan organs, with DlCOL1, DlCOL3, and DlCOL9 expressed in all organs, with the highest expression levels in floral buds. In the differential expression at different flowering induction stages of 'Sijimi' ('SJ') or 'Shixia' longan ('SX'), DlCOL4 expression was upregulated by 3-fold at the "T1-T2" flowering induction stage in 'SJ', but there was no expression during the three flowering induction stages in 'SX'. Subcellular localization analysis indicated that DlCOL4 is localized in the nucleus. Heterologous transformation of Arabidopsis indicated that DlCOL4 can negatively regulate flowering in transgenic plants. The qRT-PCR (Quantitative real-time PCR) results related to flowering genes indicated that DICOL4 may inhibit flowering by interacting with AtTFL and AtCOL. This study demonstrates the potential functional role of the DlCOL gene and the key role of DlCOL4 in regulating longan flowering.