AMOUNT OF MYOD+ CELLS IN CONNECTIVE TISSUE AT THE SITE OF DAMAGE AFTER INFRARED AND GREEN PHOTOBIOMODULATION

AIM: of study is a comparative analysis of the effects of infrared and green photobiomodulation on the number of myosatellite cells in the lesion of the damaged muscle. METHODS: 6 experimental groups were formed: control,n= 8 (0 group); I group - cut wound m. gastrocnemius (n = 40); group II - cut wound with infrared laser exposure (60 sec.) (n = 40); Group III - cut wound with infrared laser exposure (180 sec.) (n = 40); group IV – cut wound with green laser exposure (60 sec.) (n = 40); Group V - cut wound with green laser exposure (180 sec.). Laser irradiation was carried out in continuous mode with an exposure of 180 s. A morphometric analysis of histological sections stained with hematoxylin-eosin and immunohistochemical method with antibodies to the myogenic activator factorsMyoD was carried outfrom the 1st to the 30th day in the intact and focal zones. RESULTS: In the groups using infrared and green laser irradiation, an increase in the number of nuclei in the focus of damage to skeletal muscle fibers was found on the 1st, 14th and 30th day of observation. At the same time, the density ofMyoD+nucleiincreased significantly on the 1st day of the experiment. The most pronounced stimulating effect onMyoD+cells was detected after short-term irradiation with a green laser. CONCLUSION: The use of green and infrared photobiomodulation leads to an early increase in the number ofMyoD+and nuclei in skeletal muscle fibers. The most pronounced increase in the number of cells differentiating in the myogenic direction was found in animals after short-term green photobiomodulation.