Rapid and visual detection of transmissible gastroenteritis virus using a CRISPR/Cas12a system combined with loop-mediated isothermal amplification
We have successfully developed a CRISPR-Cas12a fluorescence assay and a CRISPR/Cas12a lateral-flow dipstick assay for clinical TGEV detection. Overall, we created a portable, quick, and sensitive TGEV assay with strong specificity utilizing the CRISPR-Cas12a system.
Authors
- Ying Shao (ORCID: https://orcid.org/0000-0002-0390-3315)
- Qi Dong Zhao (ORCID: https://orcid.org/0000-0001-9713-1864)
- Xiangjun Song (ORCID: https://orcid.org/0000-0002-8044-9961)
- Jiale Wang (ORCID: https://orcid.org/0000-0001-8074-4138)
- Haiyang Wang (ORCID: https://orcid.org/0000-0002-1997-1144)
- Zhe Chen (ORCID: https://orcid.org/0000-0002-5303-9879)
- Guijun Wang (ORCID: https://orcid.org/0000-0002-6294-1485)
- Liting Lu (ORCID: https://orcid.org/0000-0002-8277-5576)
- Zhenyu Wang
- Jian Tu (ORCID: https://orcid.org/0009-0005-3988-3917)
- Zhenjie He (ORCID: https://orcid.org/0009-0008-8841-1707)
Institutions
- Anhui Agricultural University (CN)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-04-02
- DOI
- https://doi.org/10.1186/s12917-025-04711-1
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61