A COMPARATIVE STUDY OF ULTRASOUND AND SUBCRITICAL WATER EXTRACTION OF PHENOLIC COMPOUNDS FROM TOBACCO WASTE

This study aimed to evaluate the potential of non-conventional techniques in extracting phenolic compounds from tobacco agro-industrial waste. Two fractions of tobacco waste obtained from Virovitički Duhani dd (solid waste and dust) were subjected to ultrasound and subcritical water in a wide temperature range, treatment times and various liquid-solid ratios to determine the changes in the content of phenolic compounds and antiradical activity. Analysis of extracts was performed using high-performance liquid chromatography and spectrophotometric methods. Results showed that UAE is preferable in phenolic compound separation. In contrast, phenolic compounds (chlorogenic acid and rutin) were degraded during SWE, which may have increased the amount of undesirable breakdown products or beneficial simple phenolic compounds.