First fine mapping of a strain of Rhizoctonia Solani AG-3, causing tobacco target spot
Rhizoctonia solani AG-3 strain XEMS25-1 was isolated from disease leaves of tobacco target spot in Enshi, Hubei Province, China. The DNA was sequenced using Pacific Biosciences Sequel II (PacBio) and Illumina NovaSeq PE150 (Nova). Data from both sequencing platforms were combined, and the de novo assembly yielded an estimated 39.4 Mb genome. Completeness of the genome examined using Benchmarking Universal SingleCopy Orthologs (BUSCO) showed that the assembly had 93.7% of the 758 genes in fungi_odb10. PHI (Pathogen Host Interactions) database analysis revealed 519 reduced virulence genes, 91 loss of pathogenicity genes, 28 hypervirulence genes and 18 effectors might be the pathogenicity-related genes in R. solani AG-3 strain XEMS25-1. These genes could be selected as the RNA-silencing targets for exploring the molecular mechanisms of R. solani AG-3 pathogenicity on tobacco.
Authors
- Lu Zheng (ORCID: https://orcid.org/0000-0002-2414-1671)
- Rubing Xu
- Tom Hsiang (ORCID: https://orcid.org/0000-0002-9549-1847)
- Ming Qiu (ORCID: https://orcid.org/0000-0001-7268-3195)
- Yanyan Li (ORCID: https://orcid.org/0000-0002-4583-1524)
- Zhao Wang (ORCID: https://orcid.org/0000-0003-2677-3207)
- Junbin Huang (ORCID: https://orcid.org/0000-0002-6571-101X)
- Pan Ma
Institutions
- Huazhong Agricultural University (CN)
- Tobacco Research Institute (CN)
- University of Guelph (CA)
Publication Details
- Journal
- BMC Genomic Data
- Published
- 2025-04-02
- DOI
- https://doi.org/10.1186/s12863-025-01315-4
- Primary Topic
- Plant Disease Resistance and Genetics
- Type
- article
- Field-Weighted Citation Impact
- 0.00