Phenotypic Diversity of Different Geographic Populations in Tupaia belangeri

Background: Phenotypic traits are crucial for studying biological systems. Tree shrews are widely used in environmental adaptation research due to their genetic diversity and phenotypic polymorphism. The measurement of its physical indicators and skull provides a new perspective for exploring the adaptive characteristics of different tree shrew populations. Methods: To elucidate the phenotypic diversity of the Tree shrew (Tupaia belangeri) and its relationship with environmental factors, the present study measured the body shape and skull indicators of 134 individuals across 12 regions. Result: The results showed that there had significant phenotypic differences between different geographic populations, with inter population variation significantly greater than intra population variation, indicating that differentiation mainly occurs between populations. Especially Daxin population (T. b. tonquinia) and the Hainan population (T. b. modesta) had larger body mass and length, whereas the Kunming population (T. b. chinensis) was relatively smaller. Environmental factor analysis revealed significant correlations between latitude, altitude, annual average temperature, atmospheric pressure and precipitation with the morphological traits of tree shrews, with altitude had the most significant impact on phenotypic differences. The clustering and principal component analysis results also confirmed the geographical differentiation of the tree shrew in morphological studies. The research findings emphasized the role of environmental factors in shaping the phenotypic diversity of the tree shrew and provide important insights into the evolution and ecological adaptation of this species, which was of great significance for biodiversity conservation and ecosystem management.