Integration of multi-omics data and deep phenotyping provides insights into responses to single and combined abiotic stress in potato

Potato (Solanum tuberosum) is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, and flooding, which are severely exacerbated by climate change. Our understanding of crop acclimation to abiotic stress, however, remains limited. Here, we present a comprehensive molecular and physiological high-throughput profiling of potato (Solanum tuberosum, cv. Désirée) under heat, drought, and waterlogging applied as single stresses or in combinations designed to mimic realistic future scenarios. Stress responses were monitored via daily phenotyping and multi-omics analyses of leaf samples comprising proteomics, targeted transcriptomics, metabolomics, and hormonomics at several timepoints during and after stress treatments. Additionally, critical metabolites of tuber samples were analyzed at the end of the stress period. We performed integrative multi-omics data analysis using a bioinformatic pipeline that we established based on machine learning and knowledge networks. Waterlogging produced the most immediate and dramatic effects on potato plants, interestingly activating ABA responses similar to drought stress. In addition, we observed distinct stress signatures at multiple molecular levels in response to heat or drought and to a combination of both. In response to all treatments, we found a downregulation of photosynthesis at different molecular levels, an accumulation of minor amino acids, and diverse stress-induced hormones. Our integrative multi-omics analysis provides global insights into plant stress responses, facilitating improved breeding strategies toward climate-adapted potato varieties.

Authors

Institutions

Publication Details

Journal
PLANT PHYSIOLOGY
Published
2025-03-28
DOI
https://doi.org/10.1093/plphys/kiaf126
Citations
28
Primary Topic
Potato Plant Research
Type
article
Field-Weighted Citation Impact
32.54

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Integration of multi-omics data and deep phenotyping provides insights into responses to single and combined abiotic stress in potato

Maja Zagorščak, Jitka Široká, Ondřej Novák, C. Bachem et al.
28 citations
PLANT PHYSIOLOGY
Potato Plant Research
32.54
article

Integration of multi-omics data and deep phenotyping provides insights into responses to single and combined abiotic stress in potato

Maja Zagorščak, Jitka Široká, Ondřej Novák, C. Bachem, Leila Afjehi‐Sadat, Jan Zrimec, Andrej Blejec, Marc R. Knight, Natalia Yaneth Rodriguez‐Granados, Palak Chaturvedi, Lamis Abdelhakim, Špela Baebler, Anže Županič, Miroslav Strnad, Carissa Bleker, Wolfram Weckwerth, Sophia Sonnewald, Lucía Sandra Pérez-Borroto, Klará Panzarová, Christian Schuy, Rashmi Sasidharan, Markus Teige, Aleš Pěnčík, Marus̆a Pompe‐Novak, Arindam Ghatak, Kristina Gruden, Bernhard Wurzinger
article en
28 citations

Abstract

Potato (Solanum tuberosum) is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, and flooding, which are severely exacerbated by climate change. Our understanding of crop acclimation to abiotic stress, however, remains limited. Here, we present a comprehensive molecular and physiological high-throughput profiling of potato (Solanum tuberosum, cv. Désirée) under heat, drought, and waterlogging applied as single stresses or in combinations designed to mimic realistic future scenarios. Stress responses were monitored via daily phenotyping and multi-omics analyses of leaf samples comprising proteomics, targeted transcriptomics, metabolomics, and hormonomics at several timepoints during and after stress treatments. Additionally, critical metabolites of tuber samples were analyzed at the end of the stress period. We performed integrative multi-omics data analysis using a bioinformatic pipeline that we established based on machine learning and knowledge networks. Waterlogging produced the most immediate and dramatic effects on potato plants, interestingly activating ABA responses similar to drought stress. In addition, we observed distinct stress signatures at multiple molecular levels in response to heat or drought and to a combination of both. In response to all treatments, we found a downregulation of photosynthesis at different molecular levels, an accumulation of minor amino acids, and diverse stress-induced hormones. Our integrative multi-omics analysis provides global insights into plant stress responses, facilitating improved breeding strategies toward climate-adapted potato varieties.

PLANT PHYSIOLOGYVol. 197(4)
University of Vienna (AT), Friedrich-Alexander-Universität Erlangen-Nürnberg (DE), Durham University (GB), University of Nova Gorica (SI), Utrecht University (NL), Research International (United States) (US), National Institute of Biology (SI), Photon Systems Instruments (Czechia) (CZ), Czech Academy of Sciences, Institute of Experimental Botany (CZ), Palacký University Olomouc (CZ), Wageningen University & Research (NL)
Javna Agencija za Raziskovalno Dejavnost RS, Horizon 2020 Framework Programme, European Regional Development Fund
Climate action
Openalex Percentile: Top 0%
Potato Plant Research
32.54
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.