Development and Validation of a Multiplex Real-Time PCR Assay for Rapid Screening of Main Carbapenemase Genes in Clinical Isolates and Surveillance Samples
The assay offers a rapid, sensitive and specific method for the screening of major carbapenemase genes, providing an effective tool for surveillance and infection control in clinical settings. The DNA extraction-free protocol converts this method into a good alternative for screening in 24/7 clinical laboratories. Further multiplexing to target other resistance genes, on demand, could add potential benefits to this laboratory-developed method.
Authors
- Francisco Javier Chamizo-López (ORCID: https://orcid.org/0000-0003-1328-1924)
- Begoña Palop-Borrás (ORCID: https://orcid.org/0000-0002-6668-9926)
- José Gutiérrez Fernández (ORCID: https://orcid.org/0000-0001-6146-9740)
- Mercedes Pérez-Ruiz (ORCID: https://orcid.org/0000-0003-4640-3107)
- José María Navarro‐Marí (ORCID: https://orcid.org/0000-0002-5227-5167)
- Ana Lara-Oya
- María Dolores Rojo-Martín (ORCID: https://orcid.org/0000-0002-3780-5764)
- Ana Belén Borrego-Alcaide
- Alba González-Hevilla (ORCID: https://orcid.org/0000-0001-7929-6497)
Institutions
- Universidad de Granada (ES)
- Hospital Regional Universitario de Málaga (ES)
- Hospital Universitario Virgen de las Nieves (ES)
- Complejo Hospitalario de Jaén (ES)
Publication Details
- Journal
- Antibiotics
- Published
- 2025-04-01
- DOI
- https://doi.org/10.3390/antibiotics14040363
- Citations
- 3
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 1.77