Transcripts from the src-1(cj293) mutant can encode a SRC-1 molecule lacking the SH2 domain in Caenorhabditis elegans

Previous studies suggest that the <i>src-1 ( cj293 )</i> mutation is an activated <i>src-1</i> allele in <i>C. elegans</i> with the potential to encode a molecule lacking the SH2 domain. <i>src-1 ( cj293 )</i> is a deletion with breakpoints in introns 3 and 5, deleting exons 4 and 5, which encode the SH2 domain. If exon 3 is spliced to exon 6, the reading frame is maintained. Here, RNA seq of <i>src-1 ( cj293 )</i> mutants showed that the exon 3 to exon 6 splice does not occur in <i>src-1 (+)</i> but is robustly present in <i>src-1 ( cj293 )</i> . Thus, <i>src-1 ( cj293 )</i> produces a transcript that can encode a SRC-1 molecular lacking the SH2 domain, which leads to overactive SRC-1 in growth cones of VD neurons during their outgrowth ( <i>i.e.</i> <i>src-1 ( cj293 )</i> might be a constitutively-active mutation).