Identification and characteristics of mutations promoting occult HBV infection by ultrasensitive HBsAg assay
The sensitivity of HBsAg detection reagents directly impacts the identification of occult hepatitis B virus (HBV) infection (OBI). This study aims to identify high-frequency OBI-related mutations in HBV surface antigen (HBsAg)-negative samples evaluated using a Fujirebio-Lumipulse ultrasensitive HBsAg assay and to investigate the implications of these mutations on the antigenicity of HBsAg, the detection capacities of various HBsAg assays, and the effects on intracellular and extracellular levels of HBsAg. Generally, our study offers a new perspective on OBI-related mutations by ultrasensitive HBsAg assay and lays the groundwork for further research on the OBI mechanism and the enhancement of HBsAg detection reagents.
Authors
- Ying Yan (ORCID: https://orcid.org/0000-0001-8556-7417)
- Lunan Wang (ORCID: https://orcid.org/0000-0003-3163-7891)
- Abudulimutailipu Nuermaimaiti
- Kaihao Feng
- Xinru Liu (ORCID: https://orcid.org/0000-0001-5427-0178)
- Huimin Ji (ORCID: https://orcid.org/0000-0001-7790-1550)
- Shi Song (ORCID: https://orcid.org/0009-0004-1303-0636)
- Zhuoqun Lu (ORCID: https://orcid.org/0009-0004-5666-6642)
- Le Chang (ORCID: https://orcid.org/0000-0002-6154-7304)
- Shana Halemubieke
- Qian Su (ORCID: https://orcid.org/0009-0001-0786-8123)
- Huizhen Sun (ORCID: https://orcid.org/0000-0001-8533-4349)
- Ling Mei
Institutions
- Academy of Medical Sciences (GB)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Beijing Hospital (CN)
- National Center for Clinical Laboratories (CN)
Publication Details
- Journal
- Journal of Clinical Microbiology
- Published
- 2025-04-14
- DOI
- https://doi.org/10.1128/jcm.02071-24
- Citations
- 6
- Primary Topic
- Hepatitis B Virus Studies
- Type
- article
- Field-Weighted Citation Impact
- 8.13
Funders
- National Natural Science Foundation of China