Evaluation of growth position and root associative microbes of Mitragyna speciosa on the production of psychoactive, mitragynine and alkaloids

Cultivation of kratom on riparian (Kapuas), is believed to increase leaf phytochemicals. The location of the best land and basis for the identification of red and green kratom is unknown. The purpose of this study was to identify species and the effect of land distance on the phytochemical of kratom leaves which were thought to be influenced by the composition of nutrients, growth inhibitor, and associative microorganisms. Materials to be used in this study include soil and leaf samples from red and green leaf bone kratom. Based on this research, it is known that kratom, which has been considered a variant, is genetically different and not a single species. Green kratom has the highest phytochemical content compared to red kratom, with the best planting is at a land distance of 244 m. Materials to be used in this study include soil and leaf samples from red and green leaf bone kratom. The highest phenol content was found in green kratom at 160 m (0.66 mgGAE g-1). The highest flavonoid was found in red kratom at 244 m (13.96 mg QE-1g-1). The highest alkaloid content was found in 244 m green kratom (21.30% g-1). Red kratom at 160 m distance had the highest antioxidant activity (70.69%). The results of associative microorganism observations showed the highest biofertilizer was red kratom at 244 m from the river, including phosphate solubilizing (2.19×105 CFU g-1), potassium solubilizing (1.6×105 CFU g-1), nitrogen fixation (1.1×105 CFU g-1), and phenol degradable (6.5×104 CFU g-1). Based on this research, it is known that kratom, which has been considered a variant, is genetically different and not a single species. Green kratom has the highest phytochemical content compared to red kratom, with the best planting is at a land distance of 244 m.