Multi-functional Ag2O-NiO composite bio-catalyst: Efficacy in breast cancer and anti-microbial properties

Silver and nickel composites are known for enhancing biocatalytic activity and have substantial potential in biomedical engineering due to their comprehensive applications. Here, we synthesized an Ag 2 O/NiO composite using the microwave hydrothermal method and characterized it by XRD, SEM, DLS, FTIR, TEM, and XPS analysis. The Ag 2 O/NiO composite catalyst was tested against the breast cancer cell line MDA-MB-231 and showed dose-dependent growth inhibition with IC 50 values of 100, 250, 500, and 1000 μg/ml −1 as determined by the MTT assay. In addition, the composite showed antimicrobial activity against Escherichia coli (MTCC 7410), Staphylococcus aureus (MTCC 7443), Fusarium oxysporum (RCMB 001004), and Aspergillus niger (RCMB 02724). These results suggest that the Ag 2 O/NiO composite is an active biocatalyst with promising antimicrobial and anticancer properties. • Ag 2 O/NiO composite shows antibacterial against Escherichia coli, Staphylococcus aureu. • Ag 2 O/NiO composite show antifungal against Aspergillus niger and Fusarium oxysporum. • Ag 2 O/NiO composite displayed potential against human breast cancer ( MDA-MB-231).