A portable, nanopore-based genotyping platform for near real-time detection of Puccinia graminis f. sp. tritici lineages and fungicide sensitivity
The newly developed Pgt Mobile And Real-time, PLant disEase (MARPLE) diagnostics platform we established, now allows precise typing of individual Pgt strains while simultaneously tracking changes in fungicide sensitivity, providing an early warning system for potential indicators of changes in the Pgt population and emerging fungicide resistance. Further integration of this Pgt MARPLE diagnostics platform into national surveillance programmes will support more informed management decisions and timely responses to Pgt disease outbreaks, helping reduce the devastating crop losses currently caused by this 'cereal killer'.
Authors
- Marta Colomer Molla (ORCID: https://orcid.org/0000-0003-1801-8121)
- Anthony C. Bryan (ORCID: https://orcid.org/0000-0003-0917-8705)
- Jemal Tola Horo (ORCID: https://orcid.org/0000-0002-7078-992X)
- Diane G. O. Saunders (ORCID: https://orcid.org/0000-0003-2847-5721)
- Oscar E. González-Navarro (ORCID: https://orcid.org/0000-0003-3676-6836)
- Yoseph Alemayehu
- David Hodson (ORCID: https://orcid.org/0000-0002-7691-0102)
- Zennah Kosgey
- Kitessa Gutu Danu
- Kimani Cyrus Ndung'u
- Loizos Savva
- Dominik Vinopal
Institutions
- John Innes Centre (GB)
- Norwich Research Park (GB)
- Kenya Agricultural and Livestock Research Organization (KE)
- Ambo University (ET)
- International Maize and Wheat Improvement Center (ET)
- Nepal Agricultural Research Council (NP)
- Ethiopian Institute of Agricultural Research (ET)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-04-01
- DOI
- https://doi.org/10.1186/s12864-025-11428-w
- Citations
- 5
- Primary Topic
- Wheat and Barley Genetics and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 8.75
Funders
- United States Agency for International Development
- Bill and Melinda Gates Foundation
- Aarhus Universitet
- Biotechnology and Biological Sciences Research Council