Preclinical Evaluation of HSP90 Noninvasive Imaging in Colorectal Cancer Diagnosis
Heat shock protein 90 (HSP90) is a powerful molecular chaperone responsible for the stability and integrity of various client proteins, including various transcription factors, kinases, and steroid hormone proteins, among others. Overexpression of HSP90 is closely related to cancer, immune diseases, and neurodegenerative diseases. However, there are few effective fluorescent probes for HSP90. Therefore, we synthesized a near-infrared fluorescent probe YQHSI-MPA-1 targeting HSP90. The probe was later structurally modified to improve the targeting specificity and accuracy in vivo as YQHSI-MPA-2. Furthermore, the application values of YQHSI-MPA-2 in situ CRC, AOM-DSS-induced CRC, and various metastasis models were evaluated. The results showed that YQHSI-MPA-2 had excellent specificity and high tumor contrast. In conclusion, YQHSI-MPA-2 is an ideal tool for tumor detection and surgical navigation.
Authors
- Cheng‐Wei Chen (ORCID: https://orcid.org/0000-0003-4807-3340)
- Zihan Wu (ORCID: https://orcid.org/0000-0002-2451-5231)
- Yueqing Gu (ORCID: https://orcid.org/0000-0003-1315-4618)
- Mengyao Zhou (ORCID: https://orcid.org/0009-0005-1951-1016)
- Xue Li (ORCID: https://orcid.org/0000-0003-4836-7808)
- Hui Wang (ORCID: https://orcid.org/0000-0003-2791-8981)
- Xiaohui Wang (ORCID: https://orcid.org/0000-0002-3415-5612)
- Qi Chang (ORCID: https://orcid.org/0000-0003-0012-3863)
- Ao Li
- Haoran Xu (ORCID: https://orcid.org/0000-0002-0213-207X)
- Zhihao Han (ORCID: https://orcid.org/0000-0003-3305-6521)
- Lenan Zou
- Yang Luo (ORCID: https://orcid.org/0000-0003-4536-3457)
Institutions
- China Pharmaceutical University (CN)
- Chinese Academy of Sciences (CN)
- Wenzhou Medical University (CN)
- Ruian People's Hospital (CN)
- State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (CN)
- Institute of Modern Physics (CN)
- State Key Laboratory of Natural Medicine
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2025-04-01
- DOI
- https://doi.org/10.1021/acs.analchem.4c05864
- Citations
- 1
- Primary Topic
- Heat shock proteins research
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- Natural Science Foundation of Jiangsu Province
- State Key Laboratory of Natural Medicines
- National Key Research and Development Program of China