Stability Indicating Reverse Phase High-Performance Liquid Chromatographic Method for the Analysis of Azelnidipine in Bulk Drug and Pharmaceutical Dosage Forms

AimObjective The aim of the study was to develop a highly sensitive precise rapid and uncomplicated high-performance reversed-phase liquid chromatographic method for the quantitative analysis of azelnidi-pine in bulk drug and pharmaceutical dosage forms.Methods This analysis utilized an Inertsil-C18 ODS. The mobile phase comprised acetonitrile and methanol in the ratio of 3070 vv respectively and was delivered at the flow rate of 1.0 mLmin and the injection volume was 20 mul. Detection was carried out by UV Spectroscopy at the wavelength of 280nm.Result The retention time of azelnidipine was found to be 6.490 min. The calibration plots showed within the concentration range of 20-80 ppm. The value of the correlation coefficient was found to be 0.9990. The test methods great accuracy was demonstrated by the finding that the intra- and inter-day variance was less than 1 showing the assay methods high precision.Conclusion The newly developed method for the quantitative analysis of azelnidipines is highly sensitive precise rapid and uncomplicated. Considering its reliable characteristics it may be employed for periodic testing in pharmaceutical and bulk drug formulations which helps with quality control in the manufacturing and assessment of azelnidipine products.