A Simple and Validated HPTLC Method for Eugenol Isolated from Syzygium Aromaticum L

Background Syzygium aromaticum L. buds are the primary source of eugenol 2-methoxy-4-prop-2-en-1-yl phenol a naturally occurring phenylpropanoid. It exhibits anticancer antioxidant antibacterial antifungal and anti-inflammatory properties. Its quantification is essential for quality control in pharmaceutical and industrial applications.Objectives This study aimed to develop and validate a rapid efficient and precise high-performance thin-layer chromatography HPTLC method for estimating eugenol isolated from S. aromaticum L. buds.Methods The structure of Eugenol was confirmed using thin-layer chromatography TLC Fourier-transform infrared spectroscopy FTIR proton nuclear magnetic resonance 1H NMR and gas chromatography-mass spectrometry GC-MS. HPTLC analysis employed a mobile phase comprising toluene ethyl acetate and glacial acetic acid in a 7.82.20.1 vvv ratio. Densitometric scanning was performed in the absorbance mode at 282 nm. The method was validated by International Conference on Harmonization ICH guidelines.Results The developed HPTLC method was validated for specificity precision linearity accuracy sensitivity and robustness.Conclusion This validated HPTLC method provides a reliable and precise approach for routine quality control of eugenol in raw materials and formulations. It ensures product quality safety and consistency in pharmaceuticals cosmetics essential oils and industrial applications. The method supports regulatory compliance and facilitates innovation in the therapeutic and commercial use of eugenol-based products.