Comparative genomic and phenotypic description of Escherichia ruysiae: a newly identified member of the gut microbiome of the domestic dog

All strains exhibited similar enzymatic activities and resistance to clindamycin, erythromycin, and metronidazole. The pangenome analysis revealed that most missing gene orthologs are related to motility followed by metabolism, including synthetases, reductases, phosphatases, permeases, transferases, and epimerases, as well as structural genes like efflux pumps and transporters. Phylogroup typing using the ClermonTyping method identified two main groups within the <i>E. ruysiae</i> species, Clade III and IV. Typical virulence genes associated with <i>E. coli</i> are absent in these strains. The multiple approaches used in this study expand our understanding of the diverse aspects of the recently described species, <i>E. ruysiae</i>.