Validation of a mouse model with Apoe gene knockout

Introduction: The research focuses on the ApoE-/- transgenic mouse model. This model enhances the understanding of the mechanisms underlying disease progression and allows for the testing of new therapeutic strategies. Materials and Methods: The study employed ApoE (-/-) knockout mice, which were bred to create cohorts of male mice. These males were divided into two equal groups, receiving either a standard diet or a high-fat diet. Analyses included lipid profile assessments, fasting glucose levels, and evaluation of aortic lesion area. Results and Discussion: The findings revealed statistically significant differences in the ages at which disease symptoms appeared between the groups. The high-fat diet induced hyperlipidemia, leading to accelerated atherosclerosis in the aorta, aligning with existing literature. Conclusion: ApoE knockout mice represent a promising genetic model for assessing therapeutic approaches to treat cardiovascular diseases. This model exhibits clear phenotypic manifestations of the disease and can be used for pharmacological correction of endothelial dysfunction.