Fabrication of gelatine/fucoidan nanogel-coated silver nanoparticles for the treatment of wound healing therapy and nursing care

Microbial infections and tissue damage by dressing removal are common problems in wound healing. In this study, gelatine/fucoidan nanogel coated with silver nanoparticles (Ag@Gel/Fu) by simple polymerization technique to assess the antibacterial potential Against human infectious pathogens and wound healing activity Against L929 fibroblast cells. The incorporation of silver nanoparticles in Ag@Gel/Fu nanogel was confirmed by UV-vis, XRD, and SEM analysis techniques. The results of the antibacterial assay revealed that the Ag@Gel/Fu nanogel showed excellent bacterial growth reduction and zone of inhibition against <i>Escherichia coli</i> and <i>Staphylococcus aureus,</i> depending upon the concentrations. In vitro wound healing results exhibit rapid regeneration of L929 cells in a short duration. It has more advantages, such as Ag@Gel/Fu, to prevent damage to outer skin tissue by infections. It is the more significant bacterial and biofilm infection control of the wound sites. The overall results confirmed that coating of biopolymer and marine polysaccharide fucoidan enhances the biological property of Ag@Gel/Fu nanogel and wound healing potential. Ag@Gel/Fu nanogel co-culture with fibroblast cells showed no cytotoxic effect after 48 h. Finally, Ag@Gel/Fu nanogel is an effective material for infected wound healing nursing care applications.