Exploring Ultrasound-Activated Chlorin e6 for Enhanced Tumor-Targeted Therapy

Sound therapy is an emerging technique used to treat cancerous cells. Chlorin e6 (Ce6), a popular photosensitizer in cancer therapy, may be promising to target deep tumors. This experiment measured the efficacy of free radical generation using an ultrasonicator bath, 96-well plates, dichlorodihydrofluores-cein diacetate (DCFH-DA), and varying amounts of phosphate-buffered saline (PBS), ascorbic acid, and horseradish peroxidase (HRP) to sound-activate Ce6. Ultrasound waves successfully generated free radicals in PBS and in ultrasonicated agar samples. However, whether Ce6 released higher levels of reactive oxygen species (ROS), a type of free radical, remains inconclusive due to ultrasound wave aggravation.