Centrifugation-Assisted Mitochondrial Transfer to Glioblastoma Stem Cells

Source: Mombo, B. N., et al. MitoCeption: transferring isolated human MSC mitochondria to glioblastoma stem cells. J. Vis. Exp. (2017) In this video, a method is demonstrated to enable the transfer of fluorescently labeled mitochondria into glioblastoma stem cells (GSCs) through centrifugation. The successful uptake and functional integration of the donor mitochondria are confirmed by detecting the fluoresce using confocal microscopy.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-03-31
DOI
https://doi.org/10.3791/23209
Primary Topic
Mitochondrial Function and Pathology
Type
article
Field-Weighted Citation Impact
0.00
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article

Centrifugation-Assisted Mitochondrial Transfer to Glioblastoma Stem Cells

Journal of Visualized Experiments
Mitochondrial Function and Pathology
article

Centrifugation-Assisted Mitochondrial Transfer to Glioblastoma Stem Cells

article en

Abstract

Source: Mombo, B. N., et al. MitoCeption: transferring isolated human MSC mitochondria to glioblastoma stem cells. J. Vis. Exp. (2017) In this video, a method is demonstrated to enable the transfer of fluorescently labeled mitochondria into glioblastoma stem cells (GSCs) through centrifugation. The successful uptake and functional integration of the donor mitochondria are confirmed by detecting the fluoresce using confocal microscopy.

Journal of Visualized Experiments
Openalex Percentile: Top 94%
Mitochondrial Function and Pathology
DOISourceOpenAlex
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Centrifugation-Assisted Mitochondrial Transfer to Glioblastoma Stem Cells · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS