Immobilizing and Patterning DNA on Simplified Protein-Free DNA-Based Lateral Flow Assays
This work presents a comprehensive study on DNA-based LFAs that completely avoids the use of proteins or pretreatment buffer. We address key fundamental challenges including optimizing buffer conditions for effective DNA immobilization on paper, understanding sequence-dependent detection, and ultimately developing patterning techniques that allow DNA patterns to appear on paper that exhibit distinct shapes depending on the introduced input sequences.
Authors
- Hyunbin Lee
- Ji Young Lee
- Hye Jin Hwang (ORCID: https://orcid.org/0000-0003-1083-5143)
- Min‐Young Lee (ORCID: https://orcid.org/0000-0003-3396-7632)
- Youngeun Kim (ORCID: https://orcid.org/0000-0002-9404-6936)
Institutions
- Seoul National University (KR)
- Korea Institute of Materials Science (KR)
Publication Details
- Journal
- ACS Omega
- Published
- 2025-03-31
- DOI
- https://doi.org/10.1021/acsomega.4c11255
- Citations
- 1
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.67
Funders
- Seoul National University
- National Research Foundation of Korea
- Korea Institute of Materials Science