Identification, pathogenicity and antifungal susceptibility of sea buckthorn fungal pathogens

This study presents a comprehensive identification and characterization of fungal pathogens that affect sea buckthorn (Hippophae rhamnoides). Utilizing microbiological methods, biochemical analyses, molecular genetic diagnostics – including PCR and DNA sequencing – and pathogenicity testing, three species of phytopathogenic fungi were identified: Aureobasidium pullulans, Didymella glomerata, and Epicoccum nigrum. The pathogenicity of these fungi towards sea buckthorn was experimentally confirmed. An evaluation of the isolates' sensitivity to six antifungal agents (terbinafine, nystatin, ketoconazole, clotrimazole, itraconazole, and fluconazole) indicated notable species-specific differences in susceptibility to fungicidal effects. The findings enhance our understanding of the etiology of fungal diseases affecting sea buckthorn and can inform the development of effective strategies for phytosanitary control and plant protection.