The Association Between Fisetin and Rutin Triggers an Enhanced Cytotoxicity in A431 and A375 Skin Cancer Cells

Background: Skin cancer is a public health problem and conventional treatments lead to multiple limitations that affect patients' well-being. Fisetin and rutin are two phytocompounds that have demonstrated versatile biological properties, including anticancer effects. Methods: A431 and A375 cells were used to evaluate the two compounds and their associative treatment. For investigation, after 24 hours of treatment, cell viability was assessed by MTT technique and cell morphology was analyzed. Results: The results indicated that the associative treatment is more potent compared to the individual treatment of the agents, producing a dose-dependent decrease in cell viability and enhancing cell dimorphologies with increasing the concentration. Conclusion: Associative treatment represents a promising approach as an alternative for skin cancer, exerting a superior effect compared to individual treatment. However, future research directions should be directed towards elucidating the mechanism of action of the FIS+RUT treatment, assessing the impact in 3D experimental models, and verifying the efficacy by incorporation into different nanoformulations.