Exercise Testing for Metabolic Flexibility: Time for Protocol Standardization

Metabolic syndrome (MetS) is a combination of risk factors that contribute to the development of many of today's chronic diseases. Rates of MetS continue to increase and it is now considered a worldwide epidemic. As with many chronic diseases it may take years for symptoms and the effects of MetS to manifest into severe health problems. Therefore, early detection is paramount A recently proposed method for the early detection of MetS is the assessment of an individual's metabolic flexibility during exercise. Metabolic flexibility is defined as the ability of the body to switch between energy substrates, primarily fats and carbohydrates, to produce energy and meet metabolic demand. This provides an indication of mitochondrial health, the possible beginning point of early insulin resistance and the development of MetS.Although there is widespread use of exercise and expired gas analysis to determine metabolic flexibility, there is no consensus on the appropriate guidelines, protocol, or interpretation of the subsequent data. Studies have used a variety of different protocols involving maximal and submaximal tests with step protocols ranging from 2 to 10 min, differences in data averaging, analysis, and stoichiometric equations, as well as variations in nutritional status of participants, and mode of exercise. This has led to considerable variation in reported results. Although the use of exercise to determine metabolic flexibility and act as a possible marker of early mitochondrial dysfunction holds significant promise, more work is required to determine the optimal protocol for clinical and research purposes.