3D-Printed Dual-Lineage Inductive Approach for Efficient Osteochondral Regeneration
Osteochondral defect regeneration is challenging due to the mismatch between cartilage and subchondral bone. We developed a functionalized scaffold replicating the natural architecture, biochemical and biomechanical environment of both tissues to promote concurrent regeneration. Our bilayered, zone-specific scaffold combines tailored materials for each tissue type: gelatin methacryloyl (GelMA), modified hyaluronic acid, and umbilical cord-derived extracellular matrix (ECM) for the cartilage layer; GelMA, placenta-derived ECM, and nano amorphous calcium phosphate for the osseous layer. Using 3D digital light-processing printing, we constructed the scaffold with spatially distributed biochemical and biomechanical signaling. This approach created dual chondro-/osteogenic microenvironments facilitating bone marrow mesenchymal stem cell differentiation. <i>In vivo</i> studies demonstrated concurrent regeneration of cartilage and subchondral bone tissues with robust integration. This 3D-printed biomimetic scaffold, featuring dual-lineage inductive properties, shows promising potential for efficient osteochondral regeneration and addresses complex tissue engineering requirements.
Authors
- Yiyang Yan (ORCID: https://orcid.org/0000-0003-4624-7669)
- Yi Hong (ORCID: https://orcid.org/0000-0002-5846-2596)
- Xudong Yao (ORCID: https://orcid.org/0000-0001-6207-8514)
- Wei Sun (ORCID: https://orcid.org/0000-0002-6448-9541)
- Yuqing Gu
- Junxin Lin (ORCID: https://orcid.org/0000-0003-1838-026X)
- Hao Gu (ORCID: https://orcid.org/0000-0003-4814-5500)
- Xinyi Ouyang (ORCID: https://orcid.org/0009-0009-6536-2815)
- Xiuying Chen (ORCID: https://orcid.org/0000-0002-7039-5410)
- Xiaozhao Wang (ORCID: https://orcid.org/0009-0002-0257-4393)
- Wei Wei (ORCID: https://orcid.org/0000-0002-2234-4459)
- Fan Zhang (ORCID: https://orcid.org/0000-0002-4480-2158)
- Shufang Zhang (ORCID: https://orcid.org/0000-0001-9488-5652)
- Rui Li (ORCID: https://orcid.org/0000-0002-0191-3050)
- Zhanfeng Cui (ORCID: https://orcid.org/0000-0002-9251-9752)
- Wenyue Li (ORCID: https://orcid.org/0000-0003-4748-982X)
- Yifei Yang (ORCID: https://orcid.org/0000-0002-6203-8552)
- Bingbing Wu (ORCID: https://orcid.org/0000-0002-0250-104X)
- Chang Xie
- Bin He (ORCID: https://orcid.org/0000-0001-8468-7492)
Institutions
- Anhui Medical University (CN)
- University of Oxford (GB)
- The Fourth People's Hospital (CN)
- Science Oxford (GB)
- Second Affiliated Hospital of Zhejiang University (CN)
- Zhejiang University-University of Edinburgh Institute (CN)
- Institute of Biomedical Science (GB)
- Centre for Advanced Study (NO)
- Fourth Affiliated Hospital of Anhui Medical University (CN)
- Zhejiang Medicine (China) (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- ACS Applied Materials & Interfaces
- Published
- 2025-03-31
- DOI
- https://doi.org/10.1021/acsami.4c14063
- Citations
- 4
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 2.70
Funders
- National Natural Science Foundation of China