SELECTIN EXPRESSION AND SEVERITY OF NITROSYLATING STRESS IN PATIENTS WITH ACUTE VENOUS THROMBOSIS OF THE LOWER EXTREMITIES

Introduction. Acute venous thrombosis is a socially significant pathology, and therefore the molecular basis of its pathogenesis is attracting close attention from modern scientists. At the same time, the adhesive function performed by CD62P, CD62E and CD62L molecules has not been sufficiently studied, especially under conditions of changes in the synthesis of nitric oxide (II). The aim. To evaluate the effect of nitric oxide on changes in the expression of intercellular adhesion molecules CD62P, CD62E and CD62L, based on markers of nitrosylating stress in the blood serum of patients with acute venous thrombosis. Material and methods. Guided by the design of the study, two groups were formed: control – 30 clinically healthy donors and experimental – 30 pa-tients with acute venous thrombosis of the lower extremities. The ELISA sandwich method was used for quantitative analysis of blood serum for CD62P, CD62E and CD62L and nitric oxide (II) synthases, the competitive ELISA method for the determination of nitrothyrosine (NT). Nitric oxide metab-olites (nitrates and nitrites) were evaluated using the Metelskaya V.A. method. The formation of bityrosine crosslinking was recorded by the intensity of fluorescent glow. Results. The concentration of CD62P in the blood serum of healthy donors and patients with acute venous thrombosis was 51.83 [35.13; 80.35] and 131.18 [110.70; 164.32], respectively (p=0.001), which was statistically significantly lower than in healthy individuals; CD62E and CD62L tended to de-crease. The concentration of nitric oxide metabolites was 43.15[33.50; 51.12] and 122.8 [71.21; 164.79], respectively (p=0.001), other markers of ni-trosylating stress showed multidirectional changes. A direct positive correlation of average strength was found between CD62L and the concentration of nitrates and nitrites. Conclusion. Nitrosylating stress affects the adhesive function of the endothelium during the formation of a blood clot in the veins, which is accompa-nied by decreased expression of CD62P, CD62E and CD62L and a multidirectional change in the concentration of marker molecules HC – nitrothyrosine and bityrosine. At the same time, an increase in NOx is noted, which acts as an auxiliary pathway for the synthesis of NO (II). The noted transfor-mations confirm the integration of nitric oxide metabolism and the expression of CD62L, CD62E and CD62L in hypercoagulation.