Microsatellite repeat mapping shows inner chromosomal diversification in highly conserved karyotypes of Asian cyprinid fishes
The barbels of the subfamilies ´Poropuntinae´ and Smiliogastrinae within the family Cyprinidae play a significant role as a food source for fish in artisanal fisheries and are highly valued as ornamental fish in Thailand. In this study, we employed both conventional and molecular cytogenetics to analyze the karyotype of 15 fish species from two cyprinid lineages. All analyzed species had a diploid chromosome number of 2n = 50. Despite sharing the same 2n, our analyses revealed species-specific distribution patterns of the mapped microsatellite motifs [(CA)₁₅, (TA)₁₅, (CAC)₁₀, and (CGG)₁₀]. They were predominantly found at telomeric sites of all-to-few chromosomes. Additionally, some species exhibited a widespread distribution of the mapped microsatellites across the chromosomes while others showed no signal. These variations reflect the evolutionary divergence and chromosomal diversity within the cyprinids. Thus, our findings support the 2n stability in cyprinoid lineages while emphasizing the intrachromosomal evolutionary diversity accompanied by species-specific microsatellite distribution.
Authors
- Alongklod Tanomtong (ORCID: https://orcid.org/0000-0002-8466-3594)
- Kriengkrai Seetapan (ORCID: https://orcid.org/0000-0003-0900-6890)
- Weerayuth Supiwong (ORCID: https://orcid.org/0000-0002-1670-3224)
- Weera Thongnetr (ORCID: https://orcid.org/0009-0000-2598-3144)
- Petr Ráb (ORCID: https://orcid.org/0000-0002-1830-3523)
- Thomas Liehr (ORCID: https://orcid.org/0000-0003-1672-3054)
- Nattasuda Donbundit (ORCID: https://orcid.org/0000-0002-3074-9288)
- Sukhonthip Ditcharoen (ORCID: https://orcid.org/0009-0009-8753-725X)
- Nawarat Muanglen (ORCID: https://orcid.org/0000-0003-4529-4252)
- Sampan Tongnunui (ORCID: https://orcid.org/0009-0002-4899-908X)
- Pasakorn Saenjundaeng
- Phichaya Buasriyot (ORCID: https://orcid.org/0000-0003-0821-7629)
- Marcelo de Bello Cioffi (ORCID: https://orcid.org/0000-0003-4340-1464)
- Sitthisak Juntharat
- Phonluang Chumpol
- Sathit Arunsang
Institutions
- Prince of Songkla University (TH)
- Universidade Federal de São Carlos (BR)
- Khon Kaen University (TH)
- Czech Academy of Sciences (CZ)
- Mahidol University (TH)
- University of Phayao (TH)
- Rajamangala University of Technology Krungthep (TH)
- Rajamangala University of Technology Suvarnabhumi (TH)
- Sakon Nakhon Rajabhat University (TH)
- Friedrich Schiller University Jena (DE)
Publication Details
- Journal
- Comparative Cytogenetics
- Published
- 2025-03-31
- DOI
- https://doi.org/10.3897/compcytogen.19.141557
- Citations
- 1
- Primary Topic
- Chromosomal and Genetic Variations
- Type
- article
- Field-Weighted Citation Impact
- 1.75
Funders
- Sakon Nakhon Rajabhat University
- Thailand Research Fund
- University of Phayao
- Royal Golden Jubilee (RGJ) Ph.D. Programme
- Thailand Science Research and Innovation