Identification of the <i>Staphylococcus aureus</i> endothelial cell surface interactome by proximity labeling

<i>Staphylococcus aureus</i> is an opportunistic pathogen that enters host cells such as epithelial or endothelial cells. Intracellular pathogens have been observed <i>in vivo</i> and are thought to serve immune evasion, avoidance of antibiotic treatment, and chronicity of infection. Thus, it is important to understand the mechanisms by which the bacteria are internalized by host cells; however, screening for pathogen-host receptors is difficult. Here, we developed a novel proximity labeling approach, which enabled the identification of several previously unknown host receptors of <i>S. aureus</i> that are engaged during a rapid uptake pathway for the bacteria.