Development and application of a blocking ELISA method based on Cap protein for detecting antibodies against porcine circovirus 2

Porcine circovirus type 2 (PCV2) has become recognized as a pathogen of significant economic concern within the swine industry. PCV2 mainly affects the immune systems of pigs, leading to a reduction in lymphocytes and resulting in immune suppression in the affected animals. Co-infection with other porcine pathogens can enhance PCV2 infection and exacerbate porcine circovirus disease. Currently, the kits available for detecting PCV2 antibodies primarily employ indirect enzyme-linked immunosorbent assay (ELISA); however, this method is prone to false positives. In contrast, the blocking ELISA method offers enhanced specificity and provides a more straightforward interpretation of results. Previous studies utilizing blocking ELISA for PCV2 antibody detection have depended on plates coated with purified PCV2 virus, a process that is both technically challenging and time-consuming. Consequently, there is a pressing need to develop a new blocking ELISA method that is more efficient to detect antibodies against PCV2.